reverse transcription Search Results


pcrbio 1 step  (PCR Biosystems Ltd)
94
PCR Biosystems Ltd pcrbio 1 step
Pcrbio 1 Step, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdna reverse transcription kit  (Vazyme Biotech Co)
96
Vazyme Biotech Co cdna reverse transcription kit
Cdna Reverse Transcription Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sybr green pcr kit  (Qiagen)
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Qiagen sybr green pcr kit
Sybr Green Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reverse transcription master mix  (fluidigm)
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fluidigm reverse transcription master mix
FIGURE 1. (A) Graphical description of treatment. An example is given of a substance to be tested in a starting solution at 10−1 M in DMSO. Targeted dilutions are 10−12, 10−10, 10−8, and 10−6 M. First, a cascade dilution in DMSO is done to obtain a 10,000 times more concentrated solution than the targeted dilution. Then 1 µL of each “mother dilution” is diluted in either 10 mL of Evian water or 5 nM T3 (prepared in Evian water). CTRL or T3 treatments are, respectively, 1 µL DMSO in 10 mL water and 1 µL DMSO in 10 mL 5nM T3. For each concentration tested, 8 mL of the prepared solution (10 mL) are pipetted into the wells and the plates are incubated at 23˚C. Daily renewal is done at a given time. After 72 h, tissues/tadpoles are collected. (B) Quantification of expression of eight typical thyroid hormone-related genes are given as examples obtained in brain tissue after 3-d exposure with T3 5 nM. Genes encoding <t>transcription</t> factors up-regulated in brain tissue after a 3-d exposure with T3 are: thyroid hormone receptor beta (thrb) with two- to threefold induction, kruppel like factor 9 (klf9) with a 10-fold induction, and thyroid hormone induced bzip factor (thibz) with a 20- to 30-fold induction. dio3 (encoding the D3 enzyme which inactivates T3 into T2) is documented to be up-regulated (two- to threefold induction) by T3 while dio1 (encoding D1) is observed to be down-regulated (twofold reduction) and dio2 (encoding the activating enzyme D2 which converts T4 into T3) remains unchanged. Two other genes encoding the nuclear receptor alpha (thra) and the membrane transporter lat2 (lat2) are documented to be down-regulated. These results are the pool of three independent experiments with n = 4 replicates in each experiment. Total RNA extraction for each replicate was obtained from two brains. 500 ng of total RNA was used for reverse transcription and 1:20 dilution cDNA was used for qPCR. See Table 1 for primers. Fold changes are presented as mean + SEM using histograms. Statistics were done using pairwise nonparametric Mann–Whitney test with (****) P < 0.0001, (**) P < 0.01, (*) P < 0.05.
Reverse Transcription Master Mix, supplied by fluidigm, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantinova reverse transcription kit  (Qiagen)
95
Qiagen quantinova reverse transcription kit
FIGURE 1. (A) Graphical description of treatment. An example is given of a substance to be tested in a starting solution at 10−1 M in DMSO. Targeted dilutions are 10−12, 10−10, 10−8, and 10−6 M. First, a cascade dilution in DMSO is done to obtain a 10,000 times more concentrated solution than the targeted dilution. Then 1 µL of each “mother dilution” is diluted in either 10 mL of Evian water or 5 nM T3 (prepared in Evian water). CTRL or T3 treatments are, respectively, 1 µL DMSO in 10 mL water and 1 µL DMSO in 10 mL 5nM T3. For each concentration tested, 8 mL of the prepared solution (10 mL) are pipetted into the wells and the plates are incubated at 23˚C. Daily renewal is done at a given time. After 72 h, tissues/tadpoles are collected. (B) Quantification of expression of eight typical thyroid hormone-related genes are given as examples obtained in brain tissue after 3-d exposure with T3 5 nM. Genes encoding <t>transcription</t> factors up-regulated in brain tissue after a 3-d exposure with T3 are: thyroid hormone receptor beta (thrb) with two- to threefold induction, kruppel like factor 9 (klf9) with a 10-fold induction, and thyroid hormone induced bzip factor (thibz) with a 20- to 30-fold induction. dio3 (encoding the D3 enzyme which inactivates T3 into T2) is documented to be up-regulated (two- to threefold induction) by T3 while dio1 (encoding D1) is observed to be down-regulated (twofold reduction) and dio2 (encoding the activating enzyme D2 which converts T4 into T3) remains unchanged. Two other genes encoding the nuclear receptor alpha (thra) and the membrane transporter lat2 (lat2) are documented to be down-regulated. These results are the pool of three independent experiments with n = 4 replicates in each experiment. Total RNA extraction for each replicate was obtained from two brains. 500 ng of total RNA was used for reverse transcription and 1:20 dilution cDNA was used for qPCR. See Table 1 for primers. Fold changes are presented as mean + SEM using histograms. Statistics were done using pairwise nonparametric Mann–Whitney test with (****) P < 0.0001, (**) P < 0.01, (*) P < 0.05.
Quantinova Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quant inova reverse transcription kit  (Qiagen)
95
Qiagen quant inova reverse transcription kit
FIGURE 1. (A) Graphical description of treatment. An example is given of a substance to be tested in a starting solution at 10−1 M in DMSO. Targeted dilutions are 10−12, 10−10, 10−8, and 10−6 M. First, a cascade dilution in DMSO is done to obtain a 10,000 times more concentrated solution than the targeted dilution. Then 1 µL of each “mother dilution” is diluted in either 10 mL of Evian water or 5 nM T3 (prepared in Evian water). CTRL or T3 treatments are, respectively, 1 µL DMSO in 10 mL water and 1 µL DMSO in 10 mL 5nM T3. For each concentration tested, 8 mL of the prepared solution (10 mL) are pipetted into the wells and the plates are incubated at 23˚C. Daily renewal is done at a given time. After 72 h, tissues/tadpoles are collected. (B) Quantification of expression of eight typical thyroid hormone-related genes are given as examples obtained in brain tissue after 3-d exposure with T3 5 nM. Genes encoding <t>transcription</t> factors up-regulated in brain tissue after a 3-d exposure with T3 are: thyroid hormone receptor beta (thrb) with two- to threefold induction, kruppel like factor 9 (klf9) with a 10-fold induction, and thyroid hormone induced bzip factor (thibz) with a 20- to 30-fold induction. dio3 (encoding the D3 enzyme which inactivates T3 into T2) is documented to be up-regulated (two- to threefold induction) by T3 while dio1 (encoding D1) is observed to be down-regulated (twofold reduction) and dio2 (encoding the activating enzyme D2 which converts T4 into T3) remains unchanged. Two other genes encoding the nuclear receptor alpha (thra) and the membrane transporter lat2 (lat2) are documented to be down-regulated. These results are the pool of three independent experiments with n = 4 replicates in each experiment. Total RNA extraction for each replicate was obtained from two brains. 500 ng of total RNA was used for reverse transcription and 1:20 dilution cDNA was used for qPCR. See Table 1 for primers. Fold changes are presented as mean + SEM using histograms. Statistics were done using pairwise nonparametric Mann–Whitney test with (****) P < 0.0001, (**) P < 0.01, (*) P < 0.05.
Quant Inova Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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omniscript reverse transcription kit  (Qiagen)
96
Qiagen omniscript reverse transcription kit
FIGURE 1. (A) Graphical description of treatment. An example is given of a substance to be tested in a starting solution at 10−1 M in DMSO. Targeted dilutions are 10−12, 10−10, 10−8, and 10−6 M. First, a cascade dilution in DMSO is done to obtain a 10,000 times more concentrated solution than the targeted dilution. Then 1 µL of each “mother dilution” is diluted in either 10 mL of Evian water or 5 nM T3 (prepared in Evian water). CTRL or T3 treatments are, respectively, 1 µL DMSO in 10 mL water and 1 µL DMSO in 10 mL 5nM T3. For each concentration tested, 8 mL of the prepared solution (10 mL) are pipetted into the wells and the plates are incubated at 23˚C. Daily renewal is done at a given time. After 72 h, tissues/tadpoles are collected. (B) Quantification of expression of eight typical thyroid hormone-related genes are given as examples obtained in brain tissue after 3-d exposure with T3 5 nM. Genes encoding <t>transcription</t> factors up-regulated in brain tissue after a 3-d exposure with T3 are: thyroid hormone receptor beta (thrb) with two- to threefold induction, kruppel like factor 9 (klf9) with a 10-fold induction, and thyroid hormone induced bzip factor (thibz) with a 20- to 30-fold induction. dio3 (encoding the D3 enzyme which inactivates T3 into T2) is documented to be up-regulated (two- to threefold induction) by T3 while dio1 (encoding D1) is observed to be down-regulated (twofold reduction) and dio2 (encoding the activating enzyme D2 which converts T4 into T3) remains unchanged. Two other genes encoding the nuclear receptor alpha (thra) and the membrane transporter lat2 (lat2) are documented to be down-regulated. These results are the pool of three independent experiments with n = 4 replicates in each experiment. Total RNA extraction for each replicate was obtained from two brains. 500 ng of total RNA was used for reverse transcription and 1:20 dilution cDNA was used for qPCR. See Table 1 for primers. Fold changes are presented as mean + SEM using histograms. Statistics were done using pairwise nonparametric Mann–Whitney test with (****) P < 0.0001, (**) P < 0.01, (*) P < 0.05.
Omniscript Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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taqman reverse transcription reagent kit  (Thermo Fisher)
90
Thermo Fisher taqman reverse transcription reagent kit
FIGURE 1. (A) Graphical description of treatment. An example is given of a substance to be tested in a starting solution at 10−1 M in DMSO. Targeted dilutions are 10−12, 10−10, 10−8, and 10−6 M. First, a cascade dilution in DMSO is done to obtain a 10,000 times more concentrated solution than the targeted dilution. Then 1 µL of each “mother dilution” is diluted in either 10 mL of Evian water or 5 nM T3 (prepared in Evian water). CTRL or T3 treatments are, respectively, 1 µL DMSO in 10 mL water and 1 µL DMSO in 10 mL 5nM T3. For each concentration tested, 8 mL of the prepared solution (10 mL) are pipetted into the wells and the plates are incubated at 23˚C. Daily renewal is done at a given time. After 72 h, tissues/tadpoles are collected. (B) Quantification of expression of eight typical thyroid hormone-related genes are given as examples obtained in brain tissue after 3-d exposure with T3 5 nM. Genes encoding <t>transcription</t> factors up-regulated in brain tissue after a 3-d exposure with T3 are: thyroid hormone receptor beta (thrb) with two- to threefold induction, kruppel like factor 9 (klf9) with a 10-fold induction, and thyroid hormone induced bzip factor (thibz) with a 20- to 30-fold induction. dio3 (encoding the D3 enzyme which inactivates T3 into T2) is documented to be up-regulated (two- to threefold induction) by T3 while dio1 (encoding D1) is observed to be down-regulated (twofold reduction) and dio2 (encoding the activating enzyme D2 which converts T4 into T3) remains unchanged. Two other genes encoding the nuclear receptor alpha (thra) and the membrane transporter lat2 (lat2) are documented to be down-regulated. These results are the pool of three independent experiments with n = 4 replicates in each experiment. Total RNA extraction for each replicate was obtained from two brains. 500 ng of total RNA was used for reverse transcription and 1:20 dilution cDNA was used for qPCR. See Table 1 for primers. Fold changes are presented as mean + SEM using histograms. Statistics were done using pairwise nonparametric Mann–Whitney test with (****) P < 0.0001, (**) P < 0.01, (*) P < 0.05.
Taqman Reverse Transcription Reagent Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantiscript reverse transcriptase kit  (Qiagen)
96
Qiagen quantiscript reverse transcriptase kit
FIGURE 1. (A) Graphical description of treatment. An example is given of a substance to be tested in a starting solution at 10−1 M in DMSO. Targeted dilutions are 10−12, 10−10, 10−8, and 10−6 M. First, a cascade dilution in DMSO is done to obtain a 10,000 times more concentrated solution than the targeted dilution. Then 1 µL of each “mother dilution” is diluted in either 10 mL of Evian water or 5 nM T3 (prepared in Evian water). CTRL or T3 treatments are, respectively, 1 µL DMSO in 10 mL water and 1 µL DMSO in 10 mL 5nM T3. For each concentration tested, 8 mL of the prepared solution (10 mL) are pipetted into the wells and the plates are incubated at 23˚C. Daily renewal is done at a given time. After 72 h, tissues/tadpoles are collected. (B) Quantification of expression of eight typical thyroid hormone-related genes are given as examples obtained in brain tissue after 3-d exposure with T3 5 nM. Genes encoding <t>transcription</t> factors up-regulated in brain tissue after a 3-d exposure with T3 are: thyroid hormone receptor beta (thrb) with two- to threefold induction, kruppel like factor 9 (klf9) with a 10-fold induction, and thyroid hormone induced bzip factor (thibz) with a 20- to 30-fold induction. dio3 (encoding the D3 enzyme which inactivates T3 into T2) is documented to be up-regulated (two- to threefold induction) by T3 while dio1 (encoding D1) is observed to be down-regulated (twofold reduction) and dio2 (encoding the activating enzyme D2 which converts T4 into T3) remains unchanged. Two other genes encoding the nuclear receptor alpha (thra) and the membrane transporter lat2 (lat2) are documented to be down-regulated. These results are the pool of three independent experiments with n = 4 replicates in each experiment. Total RNA extraction for each replicate was obtained from two brains. 500 ng of total RNA was used for reverse transcription and 1:20 dilution cDNA was used for qPCR. See Table 1 for primers. Fold changes are presented as mean + SEM using histograms. Statistics were done using pairwise nonparametric Mann–Whitney test with (****) P < 0.0001, (**) P < 0.01, (*) P < 0.05.
Quantiscript Reverse Transcriptase Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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high capacity cdna reverse transcriptase kit  (Qiagen)
96
Qiagen high capacity cdna reverse transcriptase kit
FIGURE 1. (A) Graphical description of treatment. An example is given of a substance to be tested in a starting solution at 10−1 M in DMSO. Targeted dilutions are 10−12, 10−10, 10−8, and 10−6 M. First, a cascade dilution in DMSO is done to obtain a 10,000 times more concentrated solution than the targeted dilution. Then 1 µL of each “mother dilution” is diluted in either 10 mL of Evian water or 5 nM T3 (prepared in Evian water). CTRL or T3 treatments are, respectively, 1 µL DMSO in 10 mL water and 1 µL DMSO in 10 mL 5nM T3. For each concentration tested, 8 mL of the prepared solution (10 mL) are pipetted into the wells and the plates are incubated at 23˚C. Daily renewal is done at a given time. After 72 h, tissues/tadpoles are collected. (B) Quantification of expression of eight typical thyroid hormone-related genes are given as examples obtained in brain tissue after 3-d exposure with T3 5 nM. Genes encoding <t>transcription</t> factors up-regulated in brain tissue after a 3-d exposure with T3 are: thyroid hormone receptor beta (thrb) with two- to threefold induction, kruppel like factor 9 (klf9) with a 10-fold induction, and thyroid hormone induced bzip factor (thibz) with a 20- to 30-fold induction. dio3 (encoding the D3 enzyme which inactivates T3 into T2) is documented to be up-regulated (two- to threefold induction) by T3 while dio1 (encoding D1) is observed to be down-regulated (twofold reduction) and dio2 (encoding the activating enzyme D2 which converts T4 into T3) remains unchanged. Two other genes encoding the nuclear receptor alpha (thra) and the membrane transporter lat2 (lat2) are documented to be down-regulated. These results are the pool of three independent experiments with n = 4 replicates in each experiment. Total RNA extraction for each replicate was obtained from two brains. 500 ng of total RNA was used for reverse transcription and 1:20 dilution cDNA was used for qPCR. See Table 1 for primers. Fold changes are presented as mean + SEM using histograms. Statistics were done using pairwise nonparametric Mann–Whitney test with (****) P < 0.0001, (**) P < 0.01, (*) P < 0.05.
High Capacity Cdna Reverse Transcriptase Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantifast reverse transcriptase kit  (Qiagen)
96
Qiagen quantifast reverse transcriptase kit
FIGURE 1. (A) Graphical description of treatment. An example is given of a substance to be tested in a starting solution at 10−1 M in DMSO. Targeted dilutions are 10−12, 10−10, 10−8, and 10−6 M. First, a cascade dilution in DMSO is done to obtain a 10,000 times more concentrated solution than the targeted dilution. Then 1 µL of each “mother dilution” is diluted in either 10 mL of Evian water or 5 nM T3 (prepared in Evian water). CTRL or T3 treatments are, respectively, 1 µL DMSO in 10 mL water and 1 µL DMSO in 10 mL 5nM T3. For each concentration tested, 8 mL of the prepared solution (10 mL) are pipetted into the wells and the plates are incubated at 23˚C. Daily renewal is done at a given time. After 72 h, tissues/tadpoles are collected. (B) Quantification of expression of eight typical thyroid hormone-related genes are given as examples obtained in brain tissue after 3-d exposure with T3 5 nM. Genes encoding <t>transcription</t> factors up-regulated in brain tissue after a 3-d exposure with T3 are: thyroid hormone receptor beta (thrb) with two- to threefold induction, kruppel like factor 9 (klf9) with a 10-fold induction, and thyroid hormone induced bzip factor (thibz) with a 20- to 30-fold induction. dio3 (encoding the D3 enzyme which inactivates T3 into T2) is documented to be up-regulated (two- to threefold induction) by T3 while dio1 (encoding D1) is observed to be down-regulated (twofold reduction) and dio2 (encoding the activating enzyme D2 which converts T4 into T3) remains unchanged. Two other genes encoding the nuclear receptor alpha (thra) and the membrane transporter lat2 (lat2) are documented to be down-regulated. These results are the pool of three independent experiments with n = 4 replicates in each experiment. Total RNA extraction for each replicate was obtained from two brains. 500 ng of total RNA was used for reverse transcription and 1:20 dilution cDNA was used for qPCR. See Table 1 for primers. Fold changes are presented as mean + SEM using histograms. Statistics were done using pairwise nonparametric Mann–Whitney test with (****) P < 0.0001, (**) P < 0.01, (*) P < 0.05.
Quantifast Reverse Transcriptase Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 1. (A) Graphical description of treatment. An example is given of a substance to be tested in a starting solution at 10−1 M in DMSO. Targeted dilutions are 10−12, 10−10, 10−8, and 10−6 M. First, a cascade dilution in DMSO is done to obtain a 10,000 times more concentrated solution than the targeted dilution. Then 1 µL of each “mother dilution” is diluted in either 10 mL of Evian water or 5 nM T3 (prepared in Evian water). CTRL or T3 treatments are, respectively, 1 µL DMSO in 10 mL water and 1 µL DMSO in 10 mL 5nM T3. For each concentration tested, 8 mL of the prepared solution (10 mL) are pipetted into the wells and the plates are incubated at 23˚C. Daily renewal is done at a given time. After 72 h, tissues/tadpoles are collected. (B) Quantification of expression of eight typical thyroid hormone-related genes are given as examples obtained in brain tissue after 3-d exposure with T3 5 nM. Genes encoding transcription factors up-regulated in brain tissue after a 3-d exposure with T3 are: thyroid hormone receptor beta (thrb) with two- to threefold induction, kruppel like factor 9 (klf9) with a 10-fold induction, and thyroid hormone induced bzip factor (thibz) with a 20- to 30-fold induction. dio3 (encoding the D3 enzyme which inactivates T3 into T2) is documented to be up-regulated (two- to threefold induction) by T3 while dio1 (encoding D1) is observed to be down-regulated (twofold reduction) and dio2 (encoding the activating enzyme D2 which converts T4 into T3) remains unchanged. Two other genes encoding the nuclear receptor alpha (thra) and the membrane transporter lat2 (lat2) are documented to be down-regulated. These results are the pool of three independent experiments with n = 4 replicates in each experiment. Total RNA extraction for each replicate was obtained from two brains. 500 ng of total RNA was used for reverse transcription and 1:20 dilution cDNA was used for qPCR. See Table 1 for primers. Fold changes are presented as mean + SEM using histograms. Statistics were done using pairwise nonparametric Mann–Whitney test with (****) P < 0.0001, (**) P < 0.01, (*) P < 0.05.

Journal: Cold Spring Harbor protocols

Article Title: Following Endocrine-Disrupting Effects on Gene Expression in Xenopus laevis .

doi: 10.1101/pdb.prot098301

Figure Lengend Snippet: FIGURE 1. (A) Graphical description of treatment. An example is given of a substance to be tested in a starting solution at 10−1 M in DMSO. Targeted dilutions are 10−12, 10−10, 10−8, and 10−6 M. First, a cascade dilution in DMSO is done to obtain a 10,000 times more concentrated solution than the targeted dilution. Then 1 µL of each “mother dilution” is diluted in either 10 mL of Evian water or 5 nM T3 (prepared in Evian water). CTRL or T3 treatments are, respectively, 1 µL DMSO in 10 mL water and 1 µL DMSO in 10 mL 5nM T3. For each concentration tested, 8 mL of the prepared solution (10 mL) are pipetted into the wells and the plates are incubated at 23˚C. Daily renewal is done at a given time. After 72 h, tissues/tadpoles are collected. (B) Quantification of expression of eight typical thyroid hormone-related genes are given as examples obtained in brain tissue after 3-d exposure with T3 5 nM. Genes encoding transcription factors up-regulated in brain tissue after a 3-d exposure with T3 are: thyroid hormone receptor beta (thrb) with two- to threefold induction, kruppel like factor 9 (klf9) with a 10-fold induction, and thyroid hormone induced bzip factor (thibz) with a 20- to 30-fold induction. dio3 (encoding the D3 enzyme which inactivates T3 into T2) is documented to be up-regulated (two- to threefold induction) by T3 while dio1 (encoding D1) is observed to be down-regulated (twofold reduction) and dio2 (encoding the activating enzyme D2 which converts T4 into T3) remains unchanged. Two other genes encoding the nuclear receptor alpha (thra) and the membrane transporter lat2 (lat2) are documented to be down-regulated. These results are the pool of three independent experiments with n = 4 replicates in each experiment. Total RNA extraction for each replicate was obtained from two brains. 500 ng of total RNA was used for reverse transcription and 1:20 dilution cDNA was used for qPCR. See Table 1 for primers. Fold changes are presented as mean + SEM using histograms. Statistics were done using pairwise nonparametric Mann–Whitney test with (****) P < 0.0001, (**) P < 0.01, (*) P < 0.05.

Article Snippet: Evian water in 75 cL glass bottle or another mineral water with reproducible quality High capacity cDNA RT kit (Life technologies 4368813) or Reverse Transcription Master Mix (Fluidigm 100-6299) Human chorionic gonadotropin (hCG; CAS 9002-61-3; Sigma-Aldrich CG5-1VL) Ice Liquid nitrogen Milli-Q reference water Nuclease-free water (ThermoFisher Scientific AM9937) Power SYBR Master Mix (Life Technologies 4368708) Primers, 10 pM (Eurofins; see Table 1) RNAse-free 0.8% agarose gel (optional; see Step 22) RNAqueous–micro kit (Life Technologies AM1931) RnaseZap (ThermoFisher Scientific AM9780; optional; see Step 17) Sodium hydroxide (NaOH 0.1 N; CAS 1310-73-2; optional; see Step 17) Transgenic Xenopus laevis line expressing GFP (e.g., Tg(thibz:GFP) [Fini et al. 2007]) (optional; see Step 36) 3,3′,5-Triiodo-L-thyronine sodium salt (T3) solution Xenopus laevis mature male and female (WT) or Xenopus laevis NF stage 45 tadpoles Equipment 96-well black, conical well plate (Greiner Bio-one 651209; optional; see Step 38) 384-well clear hardshell plate (ThermoFisher Scientific 4309849) Air pump and stone Adhesive covers for 384 well plates (ThermoFisher Scientific 4311971) BioAnalyzer (Agilent) Black foil to cover breeding chamber Microcentrifuge (e.g., Eppendorf 5417 R) Dissecting tools (forceps, etc.)

Techniques: Concentration Assay, Incubation, Expressing, Membrane, RNA Extraction, Reverse Transcription, MANN-WHITNEY